FastQCFastQC Report
Thu 23 Jun 2016
P1234_1020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP1234_1020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15843059
Sequences flagged as poor quality0
Sequence length123
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGA552710.34886570832059643No Hit
TACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATCGGG503520.31781741139763475No Hit
TCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGG358270.22613688429740747No Hit
TCCTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTTCTTCATCGACGC322650.20365385245362022No Hit
CTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTTCTTCATCGACGCAC291480.18397962161221518No Hit
TGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGAT270720.17087609154267494No Hit
CATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACG239560.15120817261363478No Hit
GTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATCGG232960.14704231045279828No Hit
ACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATCGGGC208070.13133196057655278No Hit
GCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGCAATTCACA199600.1259857708034793No Hit
GTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGA176660.11150624383839006No Hit
CCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGAC167840.10593913713254491No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG162390.10249914489367236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGA11200.059.579552
GTTATCG31500.055.3571133
CGGAATT31000.054.5394258
GTCGCAA19700.054.0850641
TTATCGG33250.052.4427454
GTCGGTA10900.050.4862981
TCGTTAT30500.049.713241
TCGGAAT36750.046.4833267
CGTTATC34550.046.420832
CCCGGTA48550.046.392159
ACCGTCC167550.045.565677
CACCGTC170000.045.393356
CCGTCCT170850.044.9940228
GATAGTC161700.044.7253955
TCGGTAT13200.044.3441662
CGTCCTG174350.043.3524489
GCGTACG24600.042.3601071
ATCGATT32750.042.3431175
TTATCGA32450.042.1957633
TCGCAAG25050.042.060572
Produced by FastQC (version 0.11.5)